Functional and phylogenetic analysis of prolamin proteins of foxtail millet using computational approaches

Prolamin, the alcohol-soluble seed storage protein is major in many cereals. Foxtail millet (Setaria italica) contains prolamin (Setarin) as protein, which accounts > 50% of total protein. To in-silico characterize proteins foxtail millet, sequence coding for 14.3 kDa, 20.4 22 22.4 kDa and 26 were retrieved from NCBI database. Protein physio-chemical characterization was done through Protparam found that all deficient essential amino acids like cysteine, lysine, but relatively higher amount acid, tryptophan. Isoelectric point >7 indicated basic nature low GRAVY value these hydrophobic nature. Functional by PRPSITE Pfam revealed belong to zein family prolamins. Alpha helix dominant secondary structure proteins. Multiple alignment between prolamins show series tandem repeats glutamine (Q) residues. Phylogenetic analysis Setaria viridis closely related with followed other High level homology observed signal peptide prediction cleave 21 acids. The able assess nutritional quality prolamins, addition predicting phylogenetic relationship cereal